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423 Uppsatser om Southern blot - Sida 1 av 29

Evaluation of transgenic lines of field pepperweed (Lepidium campestre L.)

The worldwide industrial demand of energy, often in form of fossil fuel, is continuously growing and at the same time these resources are coming to an end (Hamamre, 2013). The interest and focus in renewable sources are then more important than ever and plants are suggested to be a good alternative for production of raw material for industrial purposes (Nilsson et al., 1998). But the world is also facing an increasing world population which also increase the nutritional needs, plants therefore need to be suitable for both industrial and nutritional purposes. To achieve this, common plants need to be improved but it also need new potential crops to be developed and introduced (Carlsson et al., 2009). Modern technology like gene engineering is an important tool to achieve these challenges (Carlsson et al., 2011). This work focused on analyzes and evaluation of different transgenic lines of field pepperweed (Lepidium campestre L.).

Quantification of Tripeptidyl-peptidase II : Optimisation and evaluation of 3 assays

Abstract Tripeptidyl-peptidase II (TPPII), is present in most eukaryotic cells. It cuts tripeptides from the N-terminus of peptides and is especially important for degrading peptides longer than 15 amino acids. TPPII also tailors long peptides into suitable substrates for the enzymes which transport and produce the peptides that MHC I present. Increased levels of TPPII have also been found in certain cancer cells, thus it is of interest to determine if TPPII could be used as a tumour marker.The aim of this study was to optimise and evaluate 3 different methods for quantifying TPPII. Western blot, enzyme-linked immunosorbent assay (ELISA) and fluorophore-linked immunosorbent assay (FLISA) protocols were optimised regarding incubation times and antibody dilutions.

Visioner på hällar : teorin om förändrat medvetandetillstånd på sydskandinaviska hällristningar under bronsåldern

In this paper I discuss the theory of altered states of consciousness. Some of the rock art of the san-people in southern Africa and the lakota people in South Dakota, USA have been inspired by experiences during trance. The question is if some of the rock art in southern Scandinavia also have been inspired by trance experiences, and if archaeological scientists by investigating this theoretical view might find a better understanding as far as the Bronze Age in Southern Scandinavia is concerned..

Hur ska vi komma vidare? : frågor rörande neolitiseringen i Sydskandinavien

This paper deals with the question of neolithisation of Southern Scandinavia. The main goal is to give an overview of three theories regarding how agriculture and livestock breeding was introduced to the Southern Scandinavia. These are as follows: the immigration theory, the socio-economic theory and the historical explanation theory. Furthermore it is investigated which theory has the most probably support in material culture. There are obvious difficulties in interpreting the archaeological material.

Försämrad korrelation mellan storleken av Kebnekaises sydtoppsglaciär och andra klimatindikatorer

Intergovernmental Panel on Climate Change (IPCC) recognizes glaciers as the best land-indicator of climate change. A variety of studies have concluded, using both modeling and field studies, that above all climate parameters precipitation, particularly winter precipitation, and summer temperature controls the change in glacier mass balance. With global warming almost all glaciers on Earth are losing mass. In this study, the summit-glacier constituting Kebnekaise's south summit (called the Southern-summit henceforth), Sweden's highest point, is studied to see how it is affected by winter precipitation and summer temperature during the period 1968?2013.

Södra Thailand: Civila samhället - roten till våldet?

Using Varshney's theory about intra- and interethnic networks, this thesis examines how civil society in southern Thailand is built up.During 2004 the violence between Muslims and Buddhists rose in southern Thailand. The government tried to fight the insurgency using violent police and military forces, resulting in hundreds of deaths and part of reason why Thailand is loosing rank in Freedom House's democracy index. Varshney means that a civil society built up on intraethnic networks works favourable to violence between different ethnic groups. Sorting out and defining what is meant by ?ethnic? and ?civil society?, this thesis shows why to look at the insurgency in southern Thailand as an ethnic conflict surviving partly because of an intraethnic civil society.

Optimization of Western Blot for detection of cellspecific localization of DNA binding protein fromstarved cells (Dps) in Nostoc punctiforme

Cyanobacteria belong to the oldest organisms of our planet. They use photosynthesis to produce ATP and gain biomass from carbon dioxide. The cyanobacteria Nostoc punctiforme is a filamentous bacterium that consists of two different types of cells, vegetative cells and heterocysts. The type of cell it differentiates into depends on the media they grow in. In an ammonium-rich medium, the N.punctiforme consists of vegetative cells that differentiate into heterocysts when in the medium is changed to a low-concentration ammonium medium.

Movement patterns of Eurasian lynx (Lynx lynx) around their kills in southern Sweden

During the last ten- fifteen years the Eurasian lynx (Lynx lynx) population in southern Sweden has increased considerably. This area has a high density of both people and roads and have a higher roe deer (main prey of lynx) density than the rest of the country. Movement patterns such as how long animals utilize their kills and how far they travel from their kills between revisits is unknown for lynx in southern Sweden. The aim of this study was to examine how different factors affected the time lynx used their kills and the distances they travelled from the kill between revisits. The data was prepared and calculated using ArcGIS and the study is based on 98 ungulate kills and 12 lynx individuals.

De Nordeuropeiska långhögarna : Hus för de döda

The discovery and excavation of Long Barrows has been one of the major developments in the understanding of the Early Neolithic in Southern Scandinavia during the last decade. The Long Barrows are one of the oldest monumental burial forms that we see in northern Europe in the neolithic. They are also the first example of a common idea that is connected to a monumental burial form amongst the people. In this essay I attempt to show that there actually are quite a large number of Long Barrows in Scandinavia that show similarities with Long Barrows in well-known areas such as Kujavia (Poland) and Pommerania (Germany/Poland), in both architecture and location in the landscape. I also scrutinise some theories regarding this phenomenon and discuss, and i some cases, criticise them.

Prion infection of ovine cell culture with a natural Swedish scrapie isolate from 1986

Scrapie is an infectious neurologic disease in sheep caused by prions, corresponding to transmissible spongiform encephalopathies (TSEs) in other species. The prion is presumably constituted of PrPSc, the misfolded form of the normal endogenous prion protein, PrPC, which is found in practically all cells in the body. PrPSc can induce a conformational change in PrPC and misfold it as an imprint of itself; this is how prions amplify and spread. The process of conformational change is poorly understood and there might exist intermediate forms between PrPC and PrPSc. Bioassay using mice has traditionally been the golden standard in prion studies but is limited by cost and time.

El nino- Southern ocsillation och de tropiska cyklonerna i Stilla havet

ReferatDe tropiska cyklonerna har både skrämt och fascinerat människor genomtiderna och det pågår en hel del forskning kring dem. I detta arbete studeras omEl niño?Southern ocsillation påerkar antalet, intensiteten eller livslägden pådetropiska cyklonerna i Stilla havet. Till detta anväds temperaturdata frå bojarsom den amerikanska väertjästen National Oceanic and AtmosphericAdministration placerat ut i Stilla havet och insamlad information kring detropiska cyklonerna. Studien baseras pådata frå en 15ås period under åen1995 till 2009.Studien visar bland annat att antalet tropiska cykloner påerkas relativt mycketav de olika faserna i El niñ? Southern oscillation.

A literature study and survey of sycamore maple (Acer pseudoplatanus L.) in southern Sweden

Sycamore maple (Acer pseudoplatanus L.) is a common broadleaved species throughout Europe, however often of minor interest to the forest sector. There is currently a lack of research on sycamore maple, and some of its characteristics have throughout history contributed to a negative reputation in both literature and the forest society. Since broadleaved species in general are of great importance to the forest sector in Europe and presumably of enhanced significance in the future, deepened research is needed and particularly Swedish. With a desire to hopefully increase the interest for sycamore maple, was the objective of the study to examine the current status of the sycamore stands in southern Sweden. The study was carried out both as a survey with inventory field work and as a literature study. Parts of the latter mentioned is also presented in the introduction. The study shows that the majority of the sycamore stands in Sweden are growing well, producing fairly high qualities and volumes.

Sothönans ( Fulica atra ) miljökrav vid häckning i utbredningsområdets randzon

In Sweden you will find the coot chiefly in the southern parts. The species winters in the southern part of the Baltic Sea and along the coasts of northern Europe. The number of coots is heavily decreased by harsh winters. The population of the coots in Sweden is estimated to 20 000 to 30 000 pairs. The region of Dalarna is the random zone for the breeding of coots in Sweden .

Orientering av liv och död under Mellanneolitikum. : Irland, Orkneyöarna och södra England under perioden ca 4000 f.kr ? 2000 f.kr

Tovesson. R 2012: Orientering av liv och död under mellanneolitikum. Irland, Orkneyöarna och södra England under perioden ca 4000 f.kr ? 2000 f.kr.The orientation of life and death- Ireland, Orkney, and Southern England during c 4000-2000 BC.Magisteruppsats i arkeologi. Linnéuniversitetet Kalmar Vt.

Advantages and disadvantages of different observation methods on a troop of wild olive baboons at a river in Southern Kenya

Every observation method has its advantages and disadvantages and a well-adopted method gives more reliable results. Factors affecting the choice of the most appropriate methods for recording foraging behaviours include species, group size, environment, seasons and age. The focus for this study was to determine which method is most appropriate for studying the foraging behaviour of a troop of 89 olive baboons (Papio anubis) living in a riverine forest by the Southern Ewaso Ng'iro River by Lale'enok Resource Centre in Kenya. To determine this, a literature study was carried out to compare methods from monkeys and apes. I found instantaneous sampling was the most common method with intervals of 5 minutes in most cited studies.

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